Tuesday, August 30, 2016

Name _________________________          Rainbow Lab 
Date   ________________________                                               

Objectives:
1.     Demonstrate ability to follow lab procedures accurately.
2.     Develop skills for measuring liquid volumes with graduated cylinders.
3.     Follow safety procedures.
Safety:
-        Wear safety goggles at all times.
-        The liquids used in this lab can stain clothing.  Clean all spills immediately. Do not drink or taste the liquid.
-        DO NOT CROSS-CONTAMINATE any chemicals as this could cause a dangerous reaction.

Procedures:
Preparation
1.     Fill a flask halfway with water.
a.    This will be used to rinse your graduated cylinder, pipettes, and test tubes.
b.    All used rinse water should be disposed of in the “Waste Bucket” located at each table.
2.     Place 6 CLEAN test tubes in a test tube rack and label them in order A,B,C,D,E, and F.
3.     Locate three pipettes at your station.  They should be labeled “Y” (yellow), “R” (red), and “B” (blue).  These pipettes should ONLY be used to transfer the appropriate colored liquid.
4.     Rinse the graduated cylinder with water, swirl, and pour wastewater into the waste bucket.  Make sure to repeat this cleaning process between each measurement to avoid contamination.

Part A:
1.     Measure 25 mL of RED liquid and pour it into test tube A.
2.     Measure 17mL of YELLOW liquid and pour it into test tube C.
3.     Measure 21mL of BLUE liquid and pour it into test tube E.

Part B:
1.     From test tube C, measure 4 mL and pour into test tube D.
2.     From test tube E, measure 7 mL and pour into test tube D. Swirl gently.
3.     From test tube E, measure 4 mL and pour into test tube F.
4.     From test tube A, measure 7 mL and pour into test tube F. Swirl gently.
5.     From test tube A, measure 8 mL and pour into test tube B.
6.     From test tube C, measure 3 mL and pour into test tube B. Swirl gently.
7.     Obtain a score from your teacher before continuing to Part C.

Part C:
1.     Complete the data table below BEFORE cleaning up.
2.     Measure the actual amount of liquid in each tube before disposing in the waste container.
3.     Rinse all test tubes thoroughly and store each one upside down in the test tube rack to dry.

Data:
Test Tube
Description of Liquid Color
Expected Volume (mL)
Actual Volume (mL)
A



B



C



D



E



F



TOTAL Volume in Test Tubes A-F (mL)



Conclusion Questions

1.    How did your starting materials differ from your final product?  Give specific examples from the lab.


2.    What is cross-contamination and how can you prevent it from occurring?


3.    Was any liquid spilled during the lab?  What could you do to prevent chemical spills in the future?


4.    Describe and draw how to accurately read the volume of a liquid in a graduated cylinder.


5.    Scientists repeat experiments often and find the average of many data points because there is ALWAYS some amount of experimental error in any experiment.  Your goal in class is to minimize that error as much as possible.

a.    Calculate your percent error using the following equation:
                                                
                                                              Total Expected  - Total Actual
Percent Error =                 Total Expected                   x 100


          b.  What can you do in the future to minimize experimental error?